![]() ![]() This study aimed to compare the results of karyotype and the cMYC, BCL2 and BCL6 rearrangements detected by FISH performed in paraffin-embedded tissues and methanol-acetic acid fixed cells suspensions in lymph nodes samples with suspicious of NHL diagnosis.įor karyotyping, cells from lymph nodes samples were obtained after mechanical disaggregation, cultured for 24-hrs without mitogen and 72-hrs cultures with phorbol-12-myristate 13-acetate (TPA), harvested and submitted to G-banding according to standard protocols. This fresh material and methanol-acetic acid-fixed cell suspensions (arising from the karyotype preparation) could also be used for performing FISH. Although karyotype is important to determine significant abnormalities for characterization of NHL genetic subtypes, it is not used in clinical routine, because of the difficulty to send fresh material to the laboratory. 1,2,3 The standard technique for diagnosis of double or triple hit NHL is the fluorescence in situ hybridization (FISH) performed in paraffin-embedded tissue, using probes to detect cMYC (located in chromosome 8q), BCL2 (located in chromosome 18q) and BCL6 (located in chromosome 3q) rearrangements. They are categorized as a high-grade B-cell lymphoma (HGBL) according to World Health Organization (WHO) classification, have an inferior prognosis and a special therapeutic approach. Seven to 10% of DLBCL, most of them derived from germinal center cells, harbored cMYC, BLC2 and/or BCL6 translocations, known as “Double-hit” lymphoma (DHL) and/or “Triple-hit” lymphoma (THL). Diffuse large B-cell lymphomas (DLBCL) comprise a group of heterogeneous neoplasms being the most frequent subtype of non-Hodgkin Lymphoma (NHL). ![]()
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